Recombinant DNA Technology The term recombinant DNA means the joining or recombining of two pieces of DNA from two different sources. Genetic modification using recombinant DNA allows us to move genes whose function are known. By making manipulations more precise and outcomes more certain, the risk of producing organisms with unexpected traits are decreased. · Steps involved in recombinant DNA technique (or gene cloning): - · The basic 7 steps involved in gene cloning are: Isolation of DNA [gene of interest] fragments to be cloned. Insertion of isolated DNA into a suitable vector to form recombinant DNA . Introduction of recombinant DNA into a suitable organism known as host. Selection of transformed host cells and identification of the clone containing the gene of interest. Multiplication/Expression of t...